Updated 2020-04-03 by @samstudio8
So you think you are ready to provide metadata
You have read the docs?
Your data is really ready?
- You have provided at least the minimum metadata?
- Your samples have one and only one, single, unique, shareable identifier?
- You know that the “COGUK ID” does not necessarily have to be a WSI “Heron” barcode, but that it should be consistent and is usually prefixed by some short code for your site?
- Your pooled sample libraries have a name that is reasonably unique? (
BIRM-LIBRARY-001
good, 1
bad).
-
Your sequencing runs have a name that is reasonably unique? (<date>_<machine_id>_<run_no>_<some_zeros>_<flowcell>
good, my-run
bad)
- You are sure that you have permission to provide us this metadata?
-
You are sure that any identifiers are not personally identifiable?
- If multiple samples have come from the same patient, you have set the
biosample_source_id
to the first COGUK sample identifier for all of the related samples?
-
If you are re-sequencing a sample from another site, you have not changed the central_sample_id
?
- You are aware that once a record has been submitted, its
central_sample_id
(COGUK ID), library_name
and run_name
cannot be changed. If you were thinking about changing the COGUK ID, it is now or never.
-
For spreadsheet-like uploads, your data is in a CSV or XLSX with the right template for the webtool?
- It seemed to work when youn ran it on the test version of Majora? (See below)
You have uploaded data to us at least once before?
- You have registered for a test account?
- You know that this test account is different from your real CLIMB account and begins with
test-
?
- You know that the testing version of Majora has a magenta coloured banner at the top to remind you that it’s the test version?
- You have your API key token from your test profile?
- You have tried one of the two ways to upload metadata and checked it looked right on the website?
(b) You have tested the ocarina
API before?
- You have downloaded ocarina
- You know that the configration is saved to
~/.ocarina
by default and instructions for doing this appear the first time you run the tool?
- You have set the
MAJORA_DOMAIN
to https://majora-test.covid19.climb.ac.uk/
for testing?
- You set the
MAJORA_USER
and MAJORA_TOKEN
to your testing username and token?
- You have tried uploading data with:
ocarina put biosample
?
ocarina put library
?
ocarina put sequencing
?
- So you know that for
ocarina
uploads, you have to provide a biosample*, then a library, then the sequencing run, in that order? (Ask @samstudio8 about generating dummy biosamples if you want to skip straight to libraries and sequencing…).
You are ready to do this for real?
- You registered for a real unified CLIMB account and you’ve had an e-mail to tell you it was approved?
- You still know your password? (Reset)
- You have an API key token on your CLIMB profile?
- You know that this account is your real account because the username does not start with
test-
?
- You know that the API key for this account is different from the one for your test account?
-
You know that this username and API key will only work on the real Majora website?
- For webtool uploads: provide your real username and token and upload as normal
-
For ocarina
uploads: set MAJORA_DOMAIN
to https://majora.covid19.climb.ac.uk/
and update the MAJORA_USER
and MAJORA_TOKEN
to your real credentials?
- You promise to be careful to not submit test data to the real database because it’s really hard to get rid of data once it is there?
- You know that we know who has uploaded bad metadata?
- You know that without sufficient metadata, CLIMB will not publish your sample or consensus?
Published 2020-04-03. Updated 2020-04-03. Page maintainer @samstudio8.